Nature research paper: Adenylate cyclase activity of TIR1/AFB auxin receptors in plants
lines. The calcium sensor GCaMP3 was crossed to the indicated transgenic lines. Five-days-old F1 seedlings were used for vRootchip experiment, and the images were captured with a time interval of 15 s. Mock medium was changed to medium containing 500 nM of cvxIAA at 10 min. The fluorescence signal in the epidermal cells of root elongation zone was quantified, and was normalized to the average value of time points before treatment. The values shown are means + SD. n = 4 seedlings.
The experiment was repeated twice independently with similar results.line. Five-days-old seedlings of the indicated genotypes were used for vRootchip experiment. Mock medium was changed to medium containing 10 nM of IAA at 11 min. Ratiometric imaging of HPST staining was used to measure apoplastic pH in the epidermal cells of root elongation zone. The values shown are means + SD normalized to the average of those time points before treatment. n = 3 or 4 seedlings.
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