Popular pharmaceutical target in cells may prove even more useful ucsandiego nature
Left: Representative 10 μM adrenaline-stimulated yellow/cyan emission ratio changes from HEK293T cells or H9c2 cells expressing pmEKAR4, endoEKAR4, or nucEKAR4 with or without 5 μM Dyngo4a or with 10 μM ICI-118,551 preincubation. n indicates the number of cells from a representative experiment over 2 independent experiments. Right: Maximum EKAR4 responses under the indicated conditions. Solid lines in time courses indicate the mean, and shaded areas show SEM.
Left: Representative yellow/cyan emission ratio changes of HEK293T cells co-expressing EP4, D1DR, or V2R plus diffusible, endo- or nucEKAR4 upon stimulation with 10 μM PGE2, 100 μM DA or 100 nM DESMO with or without preincubation with 5 μM Dyngo4a or 100 μM barbadin. n indicates the number of cells from a representative experiment over 2 independent experiments. Right: Maximum EKAR4 responses under the indicated conditions.
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