Nature research paper: Time-resolved structural analysis of an RNA-cleaving DNA catalyst
are overlaid as a reference in each spectrum. The spectra allow to follow complex formation, effects of Na, RNA cleavage and RNA dissociation. Schematic models below spectra illustrate the respective conditions and structural features. b) Comparison of the Dz fingerprint region before and after addition of RNA. c-d) Analysis of sequential contacts occurring in NOESY spectra of free and complexed Dz . c) Schematic of analyzed sequential contacts.
g) Analysis of peak volumes representative of signal from free RNA , free Dz , and additional complex conformation . Data were acquired in the absence of NaCl and the presence of indicated concentrations of MgClconcentrations. As visible in the spectrum recorded at 100 mM NaCl and absence of MgCl, both features can also be saturated by NaCl.
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