Nature research paper: Single-cell roadmap of human gonadal development
segments before being resuspended with 1 ml of ice-cold Cryostor solution . The tissue was frozen at −80 °C by decreasing the temperature at about 1 °C per minute. The detailed protocol is available atTissue freezing
Fresh tissue samples of human developing gonads were embedded in cold optimal cutting temperature compound medium and flash frozen using a dry ice-isopentane slurry. The protocol is available at protocols.io .Developing ovaries, testes and mesonephros were collected from E10.5, E11.5 and E12.5 mouse embryos carrying the Oct4ΔPE-GFP transgene. Mice were housed in specific pathogen-free conditions at the UK Home Office-approved facility at the University of Cambridge.
Stained sections were imaged with a Perkin Elmer Opera Phenix High-Content Screening System, in confocal mode with 1 μm z-step size, using a ×20 0.16, 0.299 μm per pixel), ×40 or ×63 water-immersion objectives. Channels were as follows: DAPI , Atto 425 , Opal 520 , Opal 570 and Opal 650 .Confocal image stacks were stitched as two-dimensional maximum intensity projections using proprietary Acapella scripts provided by Perkin Elmer.
For the scATAC-seq and multimodal snRNA-seq/scATAC-seq experiments, cells were loaded according to the manufacturer’s protocol for the Chromium Single Cell ATAC v.1.0 and Chromium Single Cell Multiome ATAC + Gene Expression v.1.0 to attain between 2,000 and 10,000 cells per well. Library preparation was carried out according to the manufacturer’s protocol.
. Predicted doublets were not excluded from the initial analysis, but used afterwards to flag clusters with high doublet scores.For scRNA-seq libraries, we integrated the filtered count matrices from Cell Ranger and analysed them with Scanpy v.1.7.0, with the pipeline following their recommended standard practices.
Malaysia Latest News, Malaysia Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
For America's birthday, Mother Nature gives California a gift: cool temperatures.In a Fourth of July rarity, the weather in Southern California is nearly perfect as temperatures soar across the country.
Read more »
NatureNature is the foremost international weekly scientific journal in the world and is the flagship journal for Nature Portfolio. It publishes the finest ...
Read more »
Physicists Stunned by Nature in the Wrong Direction: Magnetic Spins That “Freeze” When HeatedPhysicists observed a very strange new type of behavior in a magnetic material when it’s heated up. When the temperature rises, the magnetic spins ‘freeze’ into a static pattern, a phenomenon that normally occurs when the temperature decreases. Their findings were published in Nature Physics today,
Read more »
Comorbidities confound Alzheimer’s blood tests - Nature MedicineThe concentrations of two key blood biomarkers for Alzheimer’s disease are affected by some medical conditions, which could potentially lead to misdiagnosis. News & Views from Suzanne Schindler and Thomas Karikari: SuzanneESchind1 TommyKakari WUSTLmed
Read more »
The observed impact of galaxy halo gas on fast radio bursts - Nature AstronomyFast radio bursts (FRBs) can be used as sensitive probes of diffuse gas in galaxy dark-matter haloes. Here the authors analyse several fast radio bursts that intersect galaxy dark-matter haloes within 40 Mpc, finding that the additional dispersion provided by the diffuse gas (presumably the intragroup medium) is in excess of 90 pc cm−3.
Read more »
The promise of precision medicine in rheumatology - Nature MedicinePrecision medicine for rheumatic diseases is still in its infancy, but recent advances are enabling comprehensive profiling and mechanistic insights. This Review outlines the progress, promises, and challenges of translating these findings into the clinic.
Read more »