Researchers have created a new assay to detect cellular protein secretion. The James Webb telescope has just been able to capture breathtaking photos of faraway galaxies that were previously only seen as hazy patches. The James Webb version for observing single-cell protein secretion has been creat
Dendritic cells are a type of immune cell that boosts immune responses.The James Webb telescope has just been able to capture breathtaking photos of faraway galaxies that were previously only seen as hazy patches. The James Webb version for observing single-cell protein secretion has been created byresearchers using a revolutionary technique that allows for stunningly detailed visualization of the proteins produced by cells.
Together with scientists from other universities and the Washington University School of Medicine, they discovered that the FluoroDOT assay is versatile, affordable, adaptable to any laboratory setting, and has the potential to provide a more comprehensive picture of these proteins than the currently commonly used assays.
This ultrabright emission of plasmonic-fluor allows the user to see extremely small quantities of secreted protein, which they are unable to do in existing assays, and measure the high-resolution signals digitally using the number of particles, or dot pattern, per cluster, or spot, using a custom-built algorithm. In addition, it doesn’t require special equipment. Singamaneni and his collaborators first published their work with the plasmonic-fluor in Nature Biomedical Engineering in 2020.
To validate the technology, the team used proteins secreted from both human and mouse cells, including immune cells infected with Mycobacterium tuberculosis.
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