Corals reveal 100-year warming history of the Pacific Ocean NatureGeosci
N are all performed at Ren lab, Department of Geosciences, National Taiwan University. The protocol follows and is modified from that of ref.. First, in an oxidative cleaning step to remove external/contaminant organic matter, 8 ml of persulfate oxidizing reagent is added to ~20 mg of coral powder in 12 ml glass vials and autoclaved at 121 °C for 1 h. The cleaning reagent is decanted, and the sample is rinsed with Milli-Q water five times and then dried in a 60 °C oven.
After oxidation, the sample is centrifuged, the clear supernatant is transferred to another precombusted 4 ml borosilicate glass vial, and the pH of the supernatant is adjusted to between 4 to 7 with HCl and NaOH. To determine the N content of the samples, we measure nitrate concentration in the oxidation solutions after autoclaving. Nitrate concentration is analysed by reduction to nitric oxide using vanadium followed by chemiluminescence detectioncorals have an average N content of 2.
. The denitrifier method involves the transformation of dissolved nitrate and nitrite into nitrous oxide gas via a naturally occurring denitrifying bacterial strain that lacks an active form of the enzyme Nwas used for this work. Normally, 5 nmol sample amounts are added to 1.5 ml of bacterial concentrate after degassing of the bacteria. Along with the samples, the organic standards and replicate analyses of nitrate reference material IAEA-NO3 , USGS-34 and a bacterial blank are also measured.
Because of the slight changes in the extension direction of this coral, we divided the whole core into 13 parts for subsampling to subsample along the maximal growth axis. We collected ~10 subsamples overlapping in time from the adjacent parts for separate analyses. We use δO and CT image to correlate the overlapping samples and to combine these parts back into a complete record.
Malaysia Latest News, Malaysia Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
In hot water: South Florida ocean tops 100 degrees; could be world recordExcessively hot waters off the coast of South Florida rose to an unfathomable level Monday evening. A buoy about 40 miles south of Miami posted a temperature of 101.1 degrees. For comparison, the “ideal” temperature of a hot tub is 100 to 102 degrees.
Read more »
South Sea scramble: Blinken, Austin lead challenge to China’s Pacific Islands cloutThe Biden administration’s push to counter China in the Indo-Pacific region will reach new heights over the coming days, with Secretary of Defense Lloyd Austin and Secretary of State Antony Blinken visiting key island nations in the South Pacific as the U.S. eyes new military and diplomatic outposts in the hotly contested region.
Read more »
Chick-fil-A plans distribution center in San Antonio areaThe Selma facility will employ more than 100 people, supporting more than 100 of its restaurants in the South Texas region.
Read more »
San Diego Comic-Con Wrap-Up: 100+ Cosplay Images: The Boys & More!Here's a look at our wrap-up image gallery (100+ photos!) of some of the best & brightest cosplay & cosplayers at SanDiegoComicCon 2023. / SDCC SDCC2023 ComicCon cosplaying
Read more »
Taylor Swift Extends Record Run Atop Artist 100, Jason Aldean & Jung Kook Hit Top 10Swift rules thanks to Speak Now (Taylor’s Version) and three other albums in the top 10.
Read more »