Nature research paper: Basis of the H2AK119 specificity of the Polycomb repressive deubiquitinase
thanks Robert Klose and the other, anonymous, reviewer for their contribution to the peer review of this work.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations., Elution profile of size-exclusion column chromatography of the BAP1–ASXL1 complex. Peak 1 was eluted close to the void volume of the column., Coomassie-stained SDS–PAGE analysis of fractions from SEC shown in .
From left to right, lane 1, no enzyme added; lanes 2–7 and lanes 8–13, successive twofold dilutions of the wild-type and C91S BAP1 enzyme complexes, respectively, detected with anti-H2A and anti-His tag antibodies., SEC-MALS analysis of BAP1 C91S–ASXL1 complex at the concentration of 2.2 mg/ml. Calculated molar mass=1648.2 kDa, which is far below the 244.2 kDa calculated molecular mass of a dimer of the BAP1–ASXL1 complex. The data were analysed by the ASTRA software.
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